Puttaraju M, Beebe J A, Niranjanakumari S, Been M D, Fierke C A
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Nucleic Acids Symp Ser. 1995(33):92-4.
A circular form of Bacillus subtilis ribonuclease P RNA (C-P RNA) was generated in vitro by splicing permuted intron-exon (PIE) sequences containing the P RNA sequence. Steady-state cleavage of pre-tRNA(Asp) catalyzed by circular P RNA is slightly faster than the linear form. Furthermore, steady-state turnover catalyzed by circular RNase P RNA is activated by the addition of the Bacillus subtilis protein component of RNase P, to a rate constant equal to the linear holoenzyme under identical conditions. Also, the circles are resistant to nuclease degradation, have less sequence heterogeneity, and may enhance the formation of a unique structure. Therefore, circular forms of RNase P RNA should prove useful for mutagenesis and structural studies.
通过剪接包含P RNA序列的置换内含子-外显子（PIE）序列，在体外产生了一种圆形的枯草芽孢杆菌核糖核酸酶P RNA（C-P RNA）。由圆形P RNA催化的前体tRNA（Asp）的稳态切割比线性形式略快。此外，通过添加核糖核酸酶P的枯草芽孢杆菌蛋白质成分，可激活由圆形核糖核酸酶P RNA催化的稳态周转，使其速率常数在相同条件下等于线性全酶。而且，这些环对核酸酶降解具有抗性，序列异质性较小，并且可能增强独特结构的形成。因此，核糖核酸酶P RNA的圆形形式应被证明对诱变和结构研究有用。
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