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Purification and radioimmunoassay of chicken growth hormone.

作者信息

Harvey S, Scanes C G

出版信息

J Endocrinol. 1977 May;73(2):321-9. doi: 10.1677/joe.0.0730321.

DOI:10.1677/joe.0.0730321
PMID:864372
Abstract

Chicken growth hormone has been isolated from adenohypophysial tissue from which the glycoprotein hormones had been removed. The procedure entailed alkali extraction, ammonium sulphate precipitation and ion-exchange chromatography on DEAE-cellulose. The resulting fraction was homogeneous, active in the rat tibia bioassay and had a similar isoelectric point, molecular weight and amino acid composition to mammalian growth hormone. A specific homologous radioimmunoassay has been developed using the avian growth hormone.

摘要

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