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大鼠肝癌细胞中S-腺苷甲硫氨酸脱羧酶基因的表达：胰岛素及蛋白质合成抑制的调节作用

S-adenosylmethionine decarboxylase gene expression in rat hepatoma cells: regulation by insulin and by inhibition of protein synthesis.

作者信息

Soininen T, Liisanantti M K, Pajunen A E

机构信息

Biocenter, University of Oulu, Finland.

出版信息

Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):273-7. doi: 10.1042/bj3160273.

DOI:10.1042/bj3160273
PMID:8645217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1217334/
Abstract

We have investigated expression of the S-adenosylmethionine decarboxylase (AdoMetDC) gene in H4-II-E rat hepatoma cells treated with growth factors (epidermal growth factor and transforming growth factor beta 1) and inducers (cAMP and insulin). Treatment with insulin caused a marked increase in both RNA level and enzyme activity. The stability of AdoMetDC mRNA was not altered by insulin treatment: the accumulation of mRNA in hepatoma cells therefore seems to be due to an increase in the transcription rate. Cycloheximide was found to be a strong inducer of AdoMetDC mRNA transcription and the effects of insulin and cycloheximide were additive, suggesting that they increase expression by separate mechanisms. Chloramphenicol acetyltransferase assays in rat hepatoma cells using 5' flanking regions of different lengths revealed that the promoter region extending 337 bp upstream from the transcription start site contains elements involved in insulin response.

摘要

我们研究了在经生长因子（表皮生长因子和转化生长因子β1）和诱导剂（cAMP和胰岛素）处理的H4-II-E大鼠肝癌细胞中S-腺苷甲硫氨酸脱羧酶（AdoMetDC）基因的表达情况。胰岛素处理导致RNA水平和酶活性均显著增加。胰岛素处理并未改变AdoMetDC mRNA的稳定性：因此，肝癌细胞中mRNA的积累似乎是由于转录速率增加所致。发现环己酰亚胺是AdoMetDC mRNA转录的强诱导剂，且胰岛素和环己酰亚胺的作用具有加和性，这表明它们通过不同机制增加表达。在大鼠肝癌细胞中使用不同长度的5'侧翼区域进行氯霉素乙酰转移酶分析表明，从转录起始位点向上游延伸337 bp的启动子区域包含参与胰岛素应答的元件。

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