Stöppler H, Kirchhoff T, Kartenbeck J, Gissmann L, Alonso A
Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Mol Carcinog. 1996 Jun;16(2):109-14. doi: 10.1002/(SICI)1098-2744(199606)16:2<109::AID-MC7>3.0.CO;2-D.
Human papillomaviruses (HPVs) are known to infect human keratinocytes and cause alterations in epithelial differentiation. We showed in this study that expression of the HPV-16 genome was able to interfere with the in vitro differentiation of a human simple-epithelial cell type, the 2102Ep teratocarcinoma cell line. Stable HPV-16 genome-expressing 2102Ep cell lines were generated, and subsequent alterations in differentiation were analyzed in comparison with parental 2102Ep cells. We found that in 2102Ep cells phorbol ester-induced differentiation led to changes in the expression of SSEA antigens, whereas in HPV-transfected cell lines only minor changes were observed.
人乳头瘤病毒（HPV）已知可感染人角质形成细胞并引起上皮分化改变。我们在本研究中表明，HPV - 16基因组的表达能够干扰一种人简单上皮细胞类型——2102Ep畸胎癌细胞系的体外分化。构建了稳定表达HPV - 16基因组的2102Ep细胞系，并与亲代2102Ep细胞相比，分析了随后的分化变化。我们发现，在2102Ep细胞中，佛波酯诱导的分化导致阶段特异性胚胎抗原（SSEA）表达发生变化，而在转染HPV的细胞系中仅观察到微小变化。
