经聚乙二醇修饰的抗体-酶缀合物的生物分布改变。
Altered biodistribution of an antibody--enzyme conjugate modified with polyethylene glycol.
作者信息
Eno-Amooquaye E A, Searle F, Boden J A, Sharma S K, Burke P J
机构信息
Department of Medical Oncology, Charing Cross Hospital, London, UK.
出版信息
Br J Cancer. 1996 Jun;73(11):1323-7. doi: 10.1038/bjc.1996.253.
Abstract
Polyethylene glycol modification of the antibody--enzyme conjugate, F(ab')2-A5B7-CPG2, extends its duration in the circulation of nude mice bearing human colonic cancer xenografts (LS174T). Increased concentration of modified conjugate is achieved in the tumour, but residual non-specific enzyme concentrations in normal tissue and blood demonstrate the fundamental requirement to remove or inactivate non-specifically held enzyme in this system.
摘要
抗体 - 酶偶联物F(ab')2 - A5B7 - CPG2的聚乙二醇修饰延长了其在携带人结肠癌异种移植瘤（LS174T）的裸鼠体内的循环时间。肿瘤中修饰后的偶联物浓度增加，但正常组织和血液中残留的非特异性酶浓度表明在该系统中去除或使非特异性结合的酶失活的基本要求。
相似文献
1
Altered biodistribution of an antibody--enzyme conjugate modified with polyethylene glycol.经聚乙二醇修饰的抗体-酶缀合物的生物分布改变。
Br J Cancer. 1996 Jun;73(11):1323-7. doi: 10.1038/bjc.1996.253.
2
Inactivation and clearance of an anti-CEA carboxypeptidase G2 conjugate in blood after localisation in a xenograft model.在异种移植模型中定位后，抗癌胚抗原羧肽酶G2缀合物在血液中的失活与清除
Br J Cancer. 1990 May;61(5):659-62. doi: 10.1038/bjc.1990.149.
3
Biodistribution of an antibody-enzyme conjugate for antibody-directed enzyme prodrug therapy in nude mice bearing a human colon adenocarcinoma xenograft.携带人结肠腺癌异种移植瘤的裸鼠体内用于抗体导向酶前药疗法的抗体-酶偶联物的生物分布
Cancer Chemother Pharmacol. 1997;40(4):277-84. doi: 10.1007/s002800050659.
4
Direct comparison of a radioiodinated intact chimeric anti-CEA MAb with its F(ab')2 fragment in nude mice bearing different human colon cancer xenografts.在携带不同人结肠癌异种移植瘤的裸鼠中，对放射性碘化的完整嵌合抗癌胚抗原单克隆抗体及其F(ab')2片段进行直接比较。
Br J Cancer. 1993 Oct;68(4):684-90. doi: 10.1038/bjc.1993.410.
5
The potential for enhanced tumour localisation by poly(ethylene glycol) modification of anti-CEA antibody.通过抗癌胚抗原（CEA）抗体的聚乙二醇修饰增强肿瘤定位的潜力。
Br J Cancer. 1994 Dec;70(6):1126-30. doi: 10.1038/bjc.1994.459.
6
Anti-tumour effects of an antibody-carboxypeptidase G2 conjugate in combination with phenol mustard prodrugs.抗体-羧肽酶G2偶联物与酚氮芥前药联合使用的抗肿瘤作用
Br J Cancer. 1995 Nov;72(5):1083-8. doi: 10.1038/bjc.1995.469.
7
Plasma clearance of an antibody--enzyme conjugate in ADEPT by monoclonal anti-enzyme: its effect on prodrug activation in vivo.单克隆抗酶在抗体导向酶解前药疗法中对抗体-酶偶联物的血浆清除：其对体内前药激活的影响
Br J Cancer. 1995 Dec;72(6):1357-63. doi: 10.1038/bjc.1995.515.
8
Preparation, characterisation and tumour targeting of cross-linked divalent and trivalent anti-tumour Fab' fragments.交联二价和三价抗肿瘤Fab'片段的制备、表征及肿瘤靶向性
Br J Cancer. 1996 Nov;74(9):1397-405. doi: 10.1038/bjc.1996.555.
9
Measurement of the critical DNA lesions produced by antibody-directed enzyme prodrug therapy (ADEPT) in vitro, in vivo and in clinical material.抗体导向酶前药疗法（ADEPT）在体外、体内及临床样本中产生的关键DNA损伤的测量。
Br J Cancer. 2001 Jun 15;84(12):1671-6. doi: 10.1054/bjoc.2001.1843.
10
Enhanced tumour specificity of an anti-carcinoembrionic antigen Fab' fragment by poly(ethylene glycol) (PEG) modification.通过聚乙二醇（PEG）修饰提高抗癌胚抗原Fab'片段的肿瘤特异性。
Br J Cancer. 1996 Jan;73(2):175-82. doi: 10.1038/bjc.1996.32.
本文引用的文献
1
Plasma clearance of an antibody--enzyme conjugate in ADEPT by monoclonal anti-enzyme: its effect on prodrug activation in vivo.单克隆抗酶在抗体导向酶解前药疗法中对抗体-酶偶联物的血浆清除：其对体内前药激活的影响
Br J Cancer. 1995 Dec;72(6):1357-63. doi: 10.1038/bjc.1995.515.
2
Optimisation of small-scale coupling of A5B7 monoclonal antibody to carboxypeptidase G2.A5B7单克隆抗体与羧肽酶G2小规模偶联的优化
J Immunol Methods. 1993 Jan 14;158(1):49-56. doi: 10.1016/0022-1759(93)90257-8.
3
Galactosylated antibodies and antibody-enzyme conjugates in antibody-directed enzyme prodrug therapy.抗体导向酶前药疗法中的半乳糖基化抗体及抗体-酶偶联物
Cancer. 1994 Feb 1;73(3 Suppl):1114-20. doi: 10.1002/1097-0142(19940201)73:3+<1114::aid-cncr2820731352>3.0.co;2-l.
4
The potential for enhanced tumour localisation by poly(ethylene glycol) modification of anti-CEA antibody.通过抗癌胚抗原（CEA）抗体的聚乙二醇修饰增强肿瘤定位的潜力。
Br J Cancer. 1994 Dec;70(6):1126-30. doi: 10.1038/bjc.1994.459.
5
Antibody directed enzyme prodrug therapy (ADEPT). A review of some theoretical, experimental and clinical aspects.抗体导向酶前药疗法（ADEPT）。对一些理论、实验和临床方面的综述。
Ann Oncol. 1994 Dec;5(10):879-91. doi: 10.1093/oxfordjournals.annonc.a058725.
6
Properties of two urate oxidases modified by the covalent attachment of poly(ethylene glycol).通过聚乙二醇共价连接修饰的两种尿酸氧化酶的性质
Biochim Biophys Acta. 1981 Aug 13;660(2):293-8. doi: 10.1016/0005-2744(81)90173-x.
8
Determination of free amino groups in proteins by trinitrobenzenesulfonic acid.用三硝基苯磺酸测定蛋白质中的游离氨基
Anal Biochem. 1966 Mar;14(3):328-36. doi: 10.1016/0003-2697(66)90275-2.
9
Surface modification of proteins. Activation of monomethoxy-polyethylene glycols by phenylchloroformates and modification of ribonuclease and superoxide dismutase.蛋白质的表面修饰。通过苯氯甲酸酯激活单甲氧基聚乙二醇并对核糖核酸酶和超氧化物歧化酶进行修饰。
Appl Biochem Biotechnol. 1985 Apr;11(2):141-52. doi: 10.1007/BF02798546.
10
Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS-16. Use of a novel triazine dye affinity method.来自假单胞菌属RS-16菌株的羧肽酶G2的纯化及性质。一种新型三嗪染料亲和法的应用。
Eur J Biochem. 1985 May 2;148(3):447-53. doi: 10.1111/j.1432-1033.1985.tb08860.x.
Zotero 插件