Kannan S, Kartha C C, Balaram P, Chandran G J, Pillai M R, Pillai K R, Nalinakumari K R, Nair M K
Regional Cancer Centre, Sree Chitra Thirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Br J Oral Maxillofac Surg. 1996 Feb;34(1):51-7. doi: 10.1016/s0266-4356(96)90136-9.
Fifteen biopsies of the immediate adjacent epithelium of oral squamous cell carcinoma were examined under light and electron microscopy. Light microscopic examination of one micron thick sections revealed that the majority of lesions (67%) had hyperplastic or mildly dysplastic epithelium while the remaining (33%) had moderate to severe dysplasia. Ultrastructural observations showed that all these lesions had subcellular alterations similar to those seen in frank malignant oral tissue, particularly in the lower half of the epithelium. Important ultrastructural changes observed included bizarre nuclei of basal and lower spinal cells, enlarged and multiple nucleoli, presence of interchromatin and perichromatin granules, loss of desmosomes and marked spongiosis as well as disturbed cellular maturation sequences in the keratinocytes evidenced by abnormal and irregular distribution of maturation markers such as keratohyalin granules and tonofilaments. The present study thus shows the value of electron microscopy in the detection of malignant changes in the adjacent epithelium of oral squamous cell carcinoma.
对15例口腔鳞状细胞癌紧邻上皮组织进行了光镜和电镜检查。对1微米厚的切片进行光镜检查发现，大多数病变（67%）为上皮增生或轻度发育异常，其余（33%）为中度至重度发育异常。超微结构观察表明，所有这些病变均有类似于口腔恶性组织中所见的亚细胞改变，尤其是上皮下半部分。观察到的重要超微结构变化包括基底细胞和下层棘细胞的怪异细胞核、核仁增大且数量增多、染色质间颗粒和核周颗粒的存在、桥粒丧失、明显的海绵形成以及角质形成细胞中细胞成熟序列紊乱，表现为透明角质颗粒和张力丝等成熟标志物分布异常和不规则。因此，本研究显示了电子显微镜在检测口腔鳞状细胞癌相邻上皮组织恶性变化中的价值。
