Manaia A C, Roitman I
J Protozool. 1977 Feb;24(1):192-5. doi: 10.1111/j.1550-7408.1977.tb05304.x.
Changes in the metabolism of Crithidia fasciculata ATCC 11745 when grown in the presence of ethidium bromide were studied. Ethidium bromide-grown cells had decreased respiratory activity as measured by oxygen consumption. More than 50% of the organisms cultivated in a defined medium containing 1.0 mg/liter of ethidium bromide became dyskinetoplastic and had decreased activities of particulate succinate and NADH-linked dehydrogenases as well as of soluble isocitrate dehydrogenase. These cells also had increased activities of particulate alpha-glycerophosphate dehydrogenase, soluble alpha-glycerophosphate dehydrogenase, malic enzyme, hexokinase, and malate dehydrogenase. Ethidium bromide-grown cells had a lower level of ATP and contained less DNA than cells grown in its absence.
研究了在溴化乙锭存在下生长时，纤细短膜虫ATCC 11745的代谢变化。通过耗氧量测定，在溴化乙锭中生长的细胞呼吸活性降低。在含有1.0毫克/升溴化乙锭的限定培养基中培养的生物体中，超过50%变成了动基体缺失型，并且颗粒状琥珀酸脱氢酶、与NADH相关的脱氢酶以及可溶性异柠檬酸脱氢酶的活性降低。这些细胞中颗粒状α-甘油磷酸脱氢酶、可溶性α-甘油磷酸脱氢酶、苹果酸酶、己糖激酶和苹果酸脱氢酶的活性也有所增加。与在无溴化乙锭条件下生长的细胞相比，在溴化乙锭中生长的细胞ATP水平较低，DNA含量也较少。
