Delarue J, Normand S, Couet C, Pachiaudi C, Urbain C, Lamisse F, Riou J P
Laboratoire de Nutrition et Jeune Equipe 313 Lipides et Croissance Hôpital Bretonneau, Tours, France.
Int J Obes Relat Metab Disord. 1996 Feb;20(2):130-6.
To study the effects of an experimental increase in plasma FFA concentration on fructose to glucose conversion, total hepatic glucose output and glycaemic response to oral fructose.
Six healthy subjects (three men, three women; age: 24.3 +/- 2.3 years; BMI: 21.6 +/- 0.8 kg/m2).
Each subject absorbed 0.5 g/kg of 13C-enriched fructose and randomly received either a triglyceride-heparin infusion or saline.
Total hepatic glucose output was traced with 6,6-2H2-glucose. Appearance in plasma of glucose synthesized from fructose was calculated from the isotopic enrichment in 13C of plasma glucose. Substrates oxidation was assessed with indirect calorimetry.
The triglycerides-heparin infusion increased FFA concentration before fructose as compared to saline (1086 +/- 40 vs 451 +/- 67 microM; p < 0.001) and lipid oxidation was 15% and 70% increased before and during fructose, respectively as compared to saline. Total hepatic glucose output, plasma appearance of glucose synthesized from fructose and glycaemic response were not affected. Glycogen storage over the first 3 h following fructose was increased (6.2 +/- 2.1 g vs 0.3 +/- 2.1 g; p < 0.01).
Triglycerides-heparin infusion did not stimulate plasma glucose appearance from fructose. Liver glucose-6-phosphate could have been produced in excess and diverted towards glycogen synthesis.
研究实验性增加血浆游离脂肪酸（FFA）浓度对果糖向葡萄糖转化、肝脏葡萄糖总输出量以及口服果糖后血糖反应的影响。
6名健康受试者（3名男性，3名女性；年龄：24.3±2.3岁；体重指数：21.6±0.8kg/m²）。
每位受试者摄入0.5g/kg的13C标记果糖，并随机接受甘油三酯-肝素输注或生理盐水输注。
用6,6-2H2-葡萄糖追踪肝脏葡萄糖总输出量。根据血浆葡萄糖中13C的同位素富集情况计算由果糖合成的葡萄糖在血浆中的出现情况。用间接测热法评估底物氧化。
与生理盐水相比，甘油三酯-肝素输注在果糖输注前增加了FFA浓度（1086±40对451±67μM；p<0.001），并且与生理盐水相比，在果糖输注前和输注期间脂质氧化分别增加了15%和70%。肝脏葡萄糖总输出量、由果糖合成的葡萄糖在血浆中的出现情况以及血糖反应均未受影响。果糖输注后最初3小时内糖原储存增加（6.2±2.1g对0.3±2.1g；p<0.01）。
甘油三酯-肝素输注并未刺激果糖生成血浆葡萄糖。肝脏中可能已过量产生6-磷酸葡萄糖并转向糖原合成。
