Das P, Meyer L, Seyfert H M, Brockmann G, Schwerin M
Forschungsinstitut für die Biologie Landwirtschaftlicher Nutztiere, Fachbereich Molekularbiologie, Dummerstorf, Germany.
Gene. 1996 Mar 9;169(2):209-13. doi: 10.1016/0378-1119(95)00815-2.
The isolation and nucleotide sequence determination of the 5' flanking region of the mouse growth hormone (mGH)-encoding gene (mGH) is described. The mGH gene consists of five exons and four introns, as is observed in other mammalian species. The second intron in mGH is much smaller than its rat counterpart, thus being similar in size to human, bovine and porcine GH. The transcription start point was determined to be a C residue 62 bp upstream from the start codon, ATG. Analysis of 1767 bp of the 5' flanking region, with respect to putative regulatory elements, revealed a TATA box, two binding sites for growth hormone factor (GHF1), a GC box (SP1), a thyroid-response element (TRE) and a silencer (SiL) sequence motif. As expected, the mGH promoter shows a higher degree of homology with rat, as compared to the other mammalian species like pig, cattle and human, where an overall homology exists only at the proximal promoter region.
本文描述了小鼠生长激素（mGH）编码基因（mGH）5'侧翼区域的分离及核苷酸序列测定。mGH基因由五个外显子和四个内含子组成，这与其他哺乳动物物种的情况一致。mGH中的第二个内含子比其大鼠对应物小得多，因此在大小上与人类、牛和猪的生长激素相似。转录起始点被确定为起始密码子ATG上游62 bp处的一个C残基。对5'侧翼区域1767 bp进行推定调控元件分析，发现了一个TATA盒、两个生长激素因子（GHF1）结合位点、一个GC盒（SP1）、一个甲状腺反应元件（TRE）和一个沉默子（SiL）序列基序。正如预期的那样，与猪、牛和人类等其他哺乳动物物种相比，mGH启动子与大鼠的同源性更高，在这些物种中，仅在近端启动子区域存在整体同源性。
