一种编码与组蛋白H2B和H3具有相似性的新型人类转录因子TFIID亚基的cDNA的分离与鉴定。

Isolation and characterization of a cDNA encoding a novel human transcription factor TFIID subunit containing similarities with histones H2B and H3.

作者信息

Choi B I, Bando M, Hasegawa S, Horikoshi M

机构信息

Laboratory of Developmental Biology, Department of Cellular Biology, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.

出版信息

Gene. 1996 Mar 9;169(2):263-7. doi: 10.1016/0378-1119(95)00838-1.

DOI:10.1016/0378-1119(95)00838-1

PMID:8647459

Abstract

Using the yeast two-hybrid system, we isolated a human cDNA that encodes a protein (hp22) interacting with TATA box-binding factor TFIID subunit p80 containing similarity with histone H4. Sequence analysis showed that the open reading frame (ORF) specifies a 161-amino-acid (aa) polypeptide homologous to Drosophila melanogaster TFIID subunit p22 (dp22). Comparison of the aa sequence of human TFIID subunit p22 (hp22) with that of dp22 revealed that p22 is composed of two distinct regions; the less conserved N-terminal (20% identity) and the highly conserved C-terminal (65% identity) regions. Additionally, the C-terminal region was found to contain similarities with histones H2B and H3. Northern blot analysis showed mRNA corresponding to hp22 to be expressed in all tissues examined.

摘要

利用酵母双杂交系统，我们分离出了一个人类cDNA，它编码一种与TATA盒结合因子TFIID亚基p80相互作用的蛋白质（hp22），该亚基与组蛋白H4存在相似性。序列分析表明，开放阅读框（ORF）编码一个与黑腹果蝇TFIID亚基p22（dp22）同源的161个氨基酸（aa）的多肽。将人类TFIID亚基p22（hp22）的氨基酸序列与dp22的氨基酸序列进行比较，发现p22由两个不同的区域组成；保守性较低的N端区域（同一性为20%）和高度保守的C端区域（同一性为65%）。此外，发现C端区域与组蛋白H2B和H3存在相似性。Northern印迹分析表明，对应于hp22的mRNA在所检测的所有组织中均有表达。