Hofmann S, Remy W, Borelli S, von Reis A, Weidinger S
Dermatologische Klinik und Poliklinik der TU, München.
Hautarzt. 1996 Mar;47(3):197-9. doi: 10.1007/s001050050402.
Tyrosinase mRNA produced by melanoma cells can be detected by reverse transcriptase-polymerase chain reaction (rt-pcr) with fine-needle tissue punctures. Fine-needle punctures from six suspected skin and lymph node metastases in three patients with malignant melanoma were analysed via rt-pcr for tyrosinase mRNA. All the suspected metastases were surgically removed and histologically examined separately. In each case the rt-pcr results and the histological diagnosis corresponded. This less invasive and highly sensitive method could prove to be a useful alternative in the diagnosis of melanoma metastasis.
通过细针组织穿刺，利用逆转录聚合酶链反应（rt-pcr）可检测到黑色素瘤细胞产生的酪氨酸酶信使核糖核酸（mRNA）。对3例恶性黑色素瘤患者的6处疑似皮肤和淋巴结转移灶进行细针穿刺，并通过rt-pcr分析酪氨酸酶mRNA。所有疑似转移灶均通过手术切除并分别进行组织学检查。在每种情况下，rt-pcr结果与组织学诊断均相符。这种侵入性较小且高度敏感的方法可能成为黑色素瘤转移诊断中一种有用的替代方法。
