Erenpreisa J, Freivalds T
Folia Histochem Cytochem (Krakow). 1979;17(1):93-8.
Rat liver, spleen and Walker carcinosarcoma imprints were subjected to depurinizing Feulgen hydrolysis and then treated with blocking agents of aldehyde groups. Such blockators as sodium bisulfite and hydroxylamine which multiplay additionally anionic groups in DNA and intensify the reactions with cationic dyes, ensuring anisotropic staining. Hydrazine lowers the binding of carionic dyes to DNA, instead phenylhydrazine, completely blocks both aldehyde and phosphate groups. When the imprints were treated with 2.4-dinitrophenylhydrazine, aldehyde and phosphate groups of apurinic acid were blocked, and DNA staining by cationic dyes occurred only on account of nitrogroups of the blocking agents which have been used. The staining reaction of cationic dyes after the use of anionogenic blocking agents of aldehyde groups is prospective not only for revealing DNA but also for several other compounds with natural or potential aldo- and ketogroups. However the reaction with phenylhydrazine can serve as a staining without removal of DNA prior to staining as an optional procedure.
对大鼠肝脏、脾脏和Walker癌肉瘤印记进行脱嘌呤Feulgen水解，然后用醛基封闭剂处理。诸如亚硫酸氢钠和羟胺之类的封闭剂会在DNA中额外引入阴离子基团，并增强与阳离子染料的反应，从而确保产生各向异性染色。肼会降低阳离子染料与DNA的结合，而苯肼则会完全封闭醛基和磷酸基团。当印记用2,4-二硝基苯肼处理时，脱嘌呤酸的醛基和磷酸基团被封闭，阳离子染料对DNA的染色仅归因于所使用封闭剂的硝基。使用醛基阴离子封闭剂后阳离子染料的染色反应不仅有望用于检测DNA，还可用于检测其他几种带有天然或潜在醛基和酮基的化合物。然而，与苯肼的反应可作为一种染色方法，无需在染色前去除DNA，这是一种可选择的操作。
