Castellani R, Parchi P, Stahl J, Capellari S, Cohen M, Gambetti P
Institute of Pathology, Division of Neuropathology, Cleveland, OH 44106, USA.
Neurology. 1996 Jun;46(6):1690-3. doi: 10.1212/wnl.46.6.1690.
We examined brain biopsy tissue from five patients with a neurologic syndrome consistent with Creutzfeldt-Jakob disease using Western blot analysis and immunohistochemistry for the detection of protease-resistant prion protein, in addition to histopathologic examination. Our results indicate that the formation of protease-resistant prion protein is an early event in disease pathogenesis and Western blot analysis can detect protease-resistant prion protein in the absence of structural lesions using a small amount of brain biopsy tissue.
