Gonzalez-Serratos H, Rozycka M, Cordoba-Rodriguez R, Ortega A
Department of Physiology, University of Maryland School of Medicine, Baltimore, 21201, USA.
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5996-6001. doi: 10.1073/pnas.93.12.5996.
In single isolated skeletal muscle fibers of the frog, we studied (i) the recovery from large sarcolemmal mechanical injuries of the response to electric stimulation and (ii) the integrity of the sarcolemma under the light microscope. In Ringer's solution, the damaged cells stopped contracting and deteriorated completely within 1 hr. In the presence of phosphatidylcholine (0.025 g/ml in Ringer's solution), the injured cells initially responded with local twitches. Within 0.5 hr, contractility and membrane integrity started to recover and both were back to control levels within 3 hr. When these cells were placed back in normal Ringer's solution, they remained viable and active for several hours. Our results suggest that phosphatidylcholine can protect muscle fibers from the effects of sarcolemmal injury.
在青蛙的单个分离骨骼肌纤维中，我们研究了：（i）对电刺激反应的肌膜大面积机械损伤后的恢复情况，以及（ii）光镜下肌膜的完整性。在任氏液中，受损细胞停止收缩，并在1小时内完全退化。在磷脂酰胆碱存在的情况下（任氏液中浓度为0.025 g/ml），受损细胞最初会出现局部抽搐反应。在0.5小时内，收缩性和膜完整性开始恢复，并在3小时内恢复到对照水平。当这些细胞重新置于正常任氏液中时，它们能保持数小时的存活和活性。我们的结果表明，磷脂酰胆碱可以保护肌纤维免受肌膜损伤的影响。
