青蛙骨骼肌纤维中的膜封闭
Membrane sealing in frog skeletal-muscle fibers.
作者信息
De Mello W C
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):982-4. doi: 10.1073/pnas.70.4.982.
Abstract
Self-sealing, not found in frog skeletal muscle fibers immersed in Ringer's solution, can be induced by solutions rich in calcium ion. Strontium replaced calcium on the sealing process, but magnesium did not. The sealing accomplished in high-calcium media was preserved in those fibers reimmersed in normal Ringer's solution. Measurements of the rate of sealing at different temperatures indicated that self-sealing induced by calcium has a high activation energy. Phospholipase C, an enzyme that hydrolyzes membrane phospholipids, produced a marked depression on the rate of sealing. Trypsin or chymotrypsin had no influence on the sealing process.
摘要
自封闭现象在浸泡于林格氏液的青蛙骨骼肌纤维中未被发现，但富含钙离子的溶液可诱导其产生。在封闭过程中，锶取代了钙，但镁没有。在高钙培养基中完成的封闭在重新浸入正常林格氏液的那些纤维中得以保留。在不同温度下对封闭速率的测量表明，钙诱导的自封闭具有较高的活化能。磷脂酶C是一种水解膜磷脂的酶，它对封闭速率有显著的抑制作用。胰蛋白酶或糜蛋白酶对封闭过程没有影响。
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