副溶血性弧菌Na+/H+逆向转运蛋白基因的克隆与测序
Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus.
作者信息
Nozaki K, Inaba K, Kuroda T, Tsuda M, Tsuchiya T
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
出版信息
Biochem Biophys Res Commun. 1996 May 24;222(3):774-9. doi: 10.1006/bbrc.1996.0820.
A gene encoding an Na+/H+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.
将编码Na⁺/H⁺逆向转运蛋白的基因从副溶血性弧菌克隆到质粒pBR322中，并在大肠杆菌细胞中表达。该基因使突变的大肠杆菌细胞能够在0.2 M NaCl（或10 mM LiCl）存在的情况下生长。这些细胞原本由于缺乏主要的Na⁺(Li⁺)/H⁺逆向转运蛋白而无法在这种条件下生长。我们在膜囊泡中检测到了该基因产生的Na⁺/H⁺逆向转运蛋白活性。对该基因进行了测序，发现推导的氨基酸序列与大肠杆菌的NhaB Na⁺/H⁺逆向转运蛋白有72%的同一性。
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