Paz D A, Ruiz G, Casco V H, Pisanó A
Laboratorio de Investigaciones Embriológicas (CONICET), Buenos Aires, Argentina.
Biocell. 1996 Apr;20(1):47-54.
Atrial natriuretic peptide (ANP)-like immunoreactivity has been demonstrated in mature spermatozoa of Bufo arenarum. However, after spermiation induced by Gonadotropin Releasing Hormone (GnRH), no ANP immunoreactivity was detected in testicular spermatozoa. Recently, the presence of GnRH and GnRH receptors in amphibian testes has been demonstrated. To clarify if the loss of ANP-like immunoreactivity in spermatozoa is a direct effect of GnRH or pituitary gonadotropins, a study on Bufo arenarum adult males, has been performed. The in vivo treatment with Human Chorionic Gonadotropin (HCG) induced spermiation and loss of ANP-like immunoreactivity. The in vitro treatment with HCG showed the same results. However, in vitro GnRH treatment failed to cause spermiation and loss of ANP-like immunoreactivity. The present results indicate that ANP from mature spermatozoa is regulated via gonadotropic hormones and may be involved in the spermiation process.
在布氏蟾蜍成熟精子中已证实存在心房利钠肽（ANP）样免疫反应性。然而，在促性腺激素释放激素（GnRH）诱导排精后，在睾丸精子中未检测到ANP免疫反应性。最近，已证实在两栖动物睾丸中存在GnRH和GnRH受体。为了阐明精子中ANP样免疫反应性的丧失是GnRH还是垂体促性腺激素的直接作用，对成年雄性布氏蟾蜍进行了一项研究。用人绒毛膜促性腺激素（HCG）进行体内治疗可诱导排精并使ANP样免疫反应性丧失。用HCG进行体外治疗也显示出相同的结果。然而，体外GnRH治疗未能引起排精和ANP样免疫反应性的丧失。目前的结果表明，成熟精子中的ANP受促性腺激素调节，可能参与排精过程。
