Zangheri E O, Labanca A M, Santana H
Departamento de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Biocell. 1996 Apr;20(1):97-103.
This study concerns the role of endogenous polyamines in the proliferation of normal hematopoietic progenitor cells: high-proliferative potential colony-forming cells (HPP-CFC), and low-proliferative potential colony-forming cells (LPP-CFC) in CFW/ep mouse bone marrow cells in the agar culture system. DL-a-difluoromethylornithine (DFMO) was used as a selective and irreversible inhibitor of ornithine decarboxylase which is the first limiting step in polyamine biosynthesis. The polyamines have been implicated in cell proliferation and differentiation. The results showed that DFMO significantly inhibited the formation of LPP-CFC colonies and completely inhibited the growth of the HPP-CFC colonies. Addition of exogenous putrescine at the concentration of 10(-7) M reverses the suppressive action of DFMO in both progenitor cells. At this concentration putrescine alone had no affect on the number or the size of the colonies. It was then concluded that endogenous polyamines appear to be essential for HPP-CFC proliferation and an important requirement for LPP-CFC proliferation.
在琼脂培养系统中CFW/ep小鼠骨髓细胞中的高增殖潜能集落形成细胞（HPP-CFC）和低增殖潜能集落形成细胞（LPP-CFC）。DL-α-二氟甲基鸟氨酸（DFMO）被用作鸟氨酸脱羧酶的选择性不可逆抑制剂，鸟氨酸脱羧酶是多胺生物合成的第一步限速酶。多胺与细胞增殖和分化有关。结果表明，DFMO显著抑制LPP-CFC集落的形成，并完全抑制HPP-CFC集落的生长。添加浓度为10^(-7) M的外源性腐胺可逆转DFMO对两种祖细胞的抑制作用。在此浓度下，单独的腐胺对集落数量或大小没有影响。由此得出结论，内源性多胺似乎是HPP-CFC增殖所必需的，也是LPP-CFC增殖的重要条件。
