来自树状嗜盐碱球菌的编码δ1-吡咯啉-5-羧酸还原酶的cDNA的分离与序列分析。

Isolation and sequence analysis of the cDNA encoding delta 1-pyrroline-5-carboxylate reductase from Zalerion arboricola.

作者信息

Kelly R, Register E

机构信息

Department of Natural Products, Merck Research Laboratories, Rahway, NJ 07065, USA.

出版信息

Gene. 1996 Jun 12;172(1):149-53. doi: 10.1016/0378-1119(96)00107-2.

DOI:10.1016/0378-1119(96)00107-2

PMID:8654976

Abstract

A cDNA encoding delta 1-pyrroline-5-carboxylate reductase (P5CR) was isolated from the pneumocandin (Pmo)-producing fungus, Zalerion arboricola (Za), by complementation of a P5CR-deficient mutant (pro3) of Saccharomyces cerevisiae (Sc). The cloned cDNA was placed under control of the Sc galactokinase (GAL1) promoter and restored P5CR activity to the pro3 mutant. Sequence analysis revealed that the Za P5CR-encoding cDNA encodes an approx. 35 kDa protein with substantial amino acid (aa) identity to P5CR from another filamentous fungus, Neurospora crassa (Nc). Za P5CR exhibits a moderate degree of aa identity to P5CR from plants, bacteria, human and Sc. This is the first gene to be isolated from Za.

摘要

通过对酿酒酵母（Sc）的脯氨酸-5-羧酸还原酶（P5CR）缺陷型突变体（pro3）进行互补，从产生肺炎链菌素（Pmo）的真菌树状枝孢霉（Za）中分离出了一个编码δ1-吡咯啉-5-羧酸还原酶（P5CR）的cDNA。将克隆的cDNA置于Sc半乳糖激酶（GAL1）启动子的控制下，恢复了pro3突变体的P5CR活性。序列分析表明，编码Za P5CR的cDNA编码一种约35 kDa的蛋白质，与另一种丝状真菌粗糙脉孢菌（Nc）的P5CR具有大量的氨基酸（aa）同一性。Za P5CR与来自植物、细菌、人类和Sc的P5CR具有中等程度的aa同一性。这是从Za中分离出的第一个基因。