Sreenivas A, Sastry P S
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Indian J Biochem Biophys. 1995 Aug;32(4):213-7.
A 100,000 x g supernatant fraction prepared from developing groundnut seeds (30-35 days after flowering) catalyzed the synthesis of fatty acids from [l-14C]acetate at a rate of 120nmoles of acetate incorporated per hr per gram fresh weight of tissue. 90% of this incorporated label was associated with fatty acids. The major fatty acids formed were stearic- (77%) and palmitic acids (14%) with 4% of oleic acid. The fatty acid synthetase activity was stable when stored at 0-4 degrees C for at least fifteen days. It is concluded from these results that acetyl-coA carboxylase and all the enzymes of fatty acid synthetase from developing groundnut seeds are soluble.
从发育中的花生种子（开花后30 - 35天）制备的100,000 x g上清液组分，以每小时每克组织鲜重掺入120纳摩尔乙酸盐的速率催化从[1 - 14C]乙酸盐合成脂肪酸。掺入的这种标记物的90%与脂肪酸相关。形成的主要脂肪酸是硬脂酸（77%）和棕榈酸（14%），油酸占4%。脂肪酸合成酶活性在0 - 4℃储存至少15天时稳定。从这些结果得出结论，发育中的花生种子中的乙酰辅酶A羧化酶和脂肪酸合成酶的所有酶都是可溶的。
