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本文引用的文献

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Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae.Gat1p是一种GATA家族蛋白，其产生对氮分解代谢阻遏敏感，参与酿酒酵母中氮分解代谢基因的转录激活。
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A small single-"finger" peptide from the erythroid transcription factor GATA-1 binds specifically to DNA as a zinc or iron complex.来自红细胞转录因子GATA-1的一种小的单“指”肽作为锌或铁复合物特异性结合DNA。
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Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.酿酒酵母中氮分解代谢基因表达对GLN3和DAL80蛋白的响应以及氮代谢物阻遏的调控回路。
J Bacteriol. 1993 Jan;175(1):64-73. doi: 10.1128/jb.175.1.64-73.1993.
4
The Saccharomyces cerevisiae DAL80 repressor protein binds to multiple copies of GATAA-containing sequences (URSGATA).酿酒酵母DAL80阻遏蛋白与多个含GATAA的序列（URSGATA）拷贝结合。
J Bacteriol. 1993 Sep;175(18):5851-61. doi: 10.1128/jb.175.18.5851-5861.1993.
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NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1.GATA-1含锌DNA结合结构域特定DNA复合物的核磁共振结构
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Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2.Cdi1，一种与Cdk2相关的人类G1期和S期蛋白磷酸酶。
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The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae.在酿酒酵母中，GLN3依赖性转录激活所需的UGA4 UASNTR位点也介导DAL80反应性调节和DAL80蛋白结合。
J Bacteriol. 1994 Aug;176(15):4718-25. doi: 10.1128/jb.176.15.4718-4725.1994.
8
The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.在酿酒酵母中，URE2蛋白通过含GATAA的UASNTR元件调节氮分解代谢基因的表达。
J Bacteriol. 1994 Dec;176(24):7476-83. doi: 10.1128/jb.176.24.7476-7483.1994.
9
Two mutually exclusive regulatory systems inhibit UASGATA, a cluster of 5'-GAT(A/T)A-3' upstream from the UGA4 gene of Saccharomyces cerevisiae.两个相互排斥的调控系统抑制UASGATA，它是酿酒酵母UGA4基因上游的一组5'-GAT(A/T)A-3'序列。
Nucleic Acids Res. 1995 Feb 25;23(4):558-64. doi: 10.1093/nar/23.4.558.
10
Roles of URE2 and GLN3 in the proline utilization pathway in Saccharomyces cerevisiae.URE2和GLN3在酿酒酵母脯氨酸利用途径中的作用。
Mol Cell Biol. 1995 Apr;15(4):2321-30. doi: 10.1128/MCB.15.4.2321.

G1n3p能够与酿酒酵母中的UAS（NTR）元件结合并激活转录。

G1n3p is capable of binding to UAS(NTR) elements and activating transcription in Saccharomyces cerevisiae.

作者信息

Cunningham T S, Svetlov V V, Rai R, Smart W, Cooper T G

机构信息

Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.

出版信息

J Bacteriol. 1996 Jun;178(12):3470-9. doi: 10.1128/jb.178.12.3470-3479.1996.

DOI:10.1128/jb.178.12.3470-3479.1996
PMID:8655543
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178115/
Abstract

When readily used nitrogen sources are available, the expression of genes encoding proteins needed to transport and metabolize poorly used nitrogen sources is repressed to low levels; this physiological response has been designated nitrogen catabolite repression (NCR). The cis-acting upstream activation sequence (UAS) element UAS(NTR) mediates Gln3p-dependent, NCR-sensitive transcription and consists of two separated dodecanucleotides, each containing the core sequence GATAA. Gln3p, produced in Escherichia coli and hence free of all other yeast proteins, specifically binds to wild-type UAS(NTR) sequences and DNA fragments derived from a variety of NCR-sensitive promoters (GDH2, CAR11 DAL3, PUT1, UGA4, and GLN1). A LexA-Gln3 fusion protein supported transcriptional activation when bound to one or more LexAp binding sites upstream of a minimal CYC1-derived promoter devoid of UAS elements. LexAp-Gln3p activation of transcription was largely independent of the nitrogen source used for growth. These data argue that Gln3p is capable of direct UAS(NTR) binding and participates in transcriptional activation of NCR-sensitive genes.

摘要

当易于利用的氮源存在时，编码转运和代谢难以利用的氮源所需蛋白质的基因表达会被抑制到低水平；这种生理反应被称为氮分解代谢物阻遏（NCR）。顺式作用上游激活序列（UAS）元件UAS(NTR)介导Gln3p依赖性、NCR敏感性转录，由两个分开的十二核苷酸组成，每个都含有核心序列GATAA。在大肠杆菌中产生的、因此不含所有其他酵母蛋白的Gln3p，特异性结合野生型UAS(NTR)序列以及源自多种NCR敏感性启动子（GDH2、CAR11、DAL3、PUT1、UGA4和GLN1）的DNA片段。当与不含UAS元件的最小CYC1衍生启动子上游的一个或多个LexAp结合位点结合时，LexA-Gln3融合蛋白支持转录激活。LexAp-Gln3p的转录激活在很大程度上不依赖于用于生长的氮源。这些数据表明Gln3p能够直接结合UAS(NTR)并参与NCR敏感性基因的转录激活。