Tieman S, Koch A, White D
Program in Microbiology, Department of Biology, Indiana University, Bloomington 47405-6801, USA.
J Bacteriol. 1996 Jun;178(12):3480-5. doi: 10.1128/jb.178.12.3480-3485.1996.
A method was devised to construct stable and steep chemical gradients in slide cultures to study the movements of gliding cells. The movement of Myxococcus xanthus individual cells and small swarms was studied in these gradients. There was no response to gradients of Casitone and yeast extract that were previously reported to stimulate a positive chemotactic response with M. xanthus.
设计了一种方法,用于在玻片培养物中构建稳定且陡峭的化学梯度,以研究滑行细胞的运动。在这些梯度中研究了黄色黏球菌单个细胞和小群体的运动。对于先前报道能刺激黄色黏球菌产生正向趋化反应的酪蛋白氨基酸和酵母提取物的梯度,细胞没有反应。