通过巢式聚合酶链反应和免疫组织化学技术对石蜡切片中的淋巴瘤进行诊断。
Diagnosis of lymphoma in paraffin wax sections by nested PCR and immunohistochemistry.
作者信息
Kitamura Y, Nanba E, Inui S, Tanigawa T, Ichihara K
机构信息
Second Department of Pathology, Tottori University Faculty of Medicine, Yonago, Japan.
出版信息
J Clin Pathol. 1996 Apr;49(4):333-7. doi: 10.1136/jcp.49.4.333.
AIMS
To investigate whether nested polymerase chain reaction (PCR) and immunohistochemistry can be used to diagnose malignant lymphoma.
METHODS
Paraffin wax embedded tissue sections from 31 patients with malignant lymphoma were analysed by nested PCR and immunohistochemistry using standard protocols.
RESULTS
Nested PCR amplification of 1 pg DNA confirmed monoclonality in B cell lymphoma; PCR amplification of 10 pg DNA confirmed monoclonality in T cell lymphoma. Twenty seven (87%) samples were diagnosed as malignant lymphoma by nested PCR, and 24 (77%) by immunohistochemistry. Seven samples were diagnosed as malignant lymphoma by nested PCR, but not by immunohistochemistry, whereas the use of both procedures gave a diagnosis of malignant lymphoma in all 31 samples.
CONCLUSIONS
A combination of immunohistochemistry and nested PCR can be used to diagnose malignant lymphoma in routine paraffin wax embedded sections.
目的
研究巢式聚合酶链反应（PCR）和免疫组织化学是否可用于诊断恶性淋巴瘤。
方法
采用标准方案，通过巢式PCR和免疫组织化学对31例恶性淋巴瘤患者的石蜡包埋组织切片进行分析。
结果
1 pg DNA的巢式PCR扩增证实B细胞淋巴瘤为单克隆性；10 pg DNA的PCR扩增证实T细胞淋巴瘤为单克隆性。27份（87%）样本通过巢式PCR诊断为恶性淋巴瘤，24份（77%）通过免疫组织化学诊断为恶性淋巴瘤。7份样本通过巢式PCR诊断为恶性淋巴瘤，但免疫组织化学未诊断出，而两种方法联合使用可使所有31份样本均诊断为恶性淋巴瘤。
结论
免疫组织化学和巢式PCR联合应用可用于常规石蜡包埋切片中恶性淋巴瘤的诊断。
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