Kornelisse R F, Hoekman K, Visser J J, Hop W C, Huijmans J G, van der Straaten P J, van der Heijden A J, Sukhai R N, Neijens H J, de Groot R
Department of Pediatrics, Sophia Children's Hospital/University Hospital, Rotterdam, Netherlands.
J Infect Dis. 1996 Jul;174(1):120-6. doi: 10.1093/infdis/174.1.120.
To investigate the role of nitric oxide (NO) in bacterial meningitis, concentrations in serum, cerebrospinal fluid (CSF), or both of the precursor (L-arginine) and degradation products of NO (nitrate, nitrite) and tumor necrosis factor (TNF)-alpha were measured in 35 patients and 30 controls. CSF nitrate levels were significantly elevated, mainly due to increased blood-brain barrier permeability, and are therefore not a good parameter for gauging endogenous NO production in the CSF compartment. CSF NO/nitrite levels were significantly elevated in patients. NO/nitrite levels decreased over time (26%/6 h; P < .001). CSF levels of NO/nitrite correlated with those of TNF-alpha (r = .55; P = .001) and glucose (r = -.43; P = .02). CSF levels of L-arginine were lower in patients than in controls (P < .001). Dexamethasone did not exert a significant effect on NO metabolism. In conclusion, enhanced NO production may contribute to anaerobic glycolysis and neurologic damage in bacterial meningitis.
为研究一氧化氮（NO）在细菌性脑膜炎中的作用，我们检测了35例患者和30例对照者血清、脑脊液（CSF）或两者中NO的前体（L-精氨酸）、NO降解产物（硝酸盐、亚硝酸盐）及肿瘤坏死因子（TNF）-α的浓度。脑脊液硝酸盐水平显著升高，主要是由于血脑屏障通透性增加，因此它并非衡量脑脊液中内源性NO生成的良好指标。患者脑脊液中NO/亚硝酸盐水平显著升高。NO/亚硝酸盐水平随时间下降（26%/6小时；P <.001）。脑脊液中NO/亚硝酸盐水平与TNF-α水平相关（r =.55；P =.001），与葡萄糖水平相关（r = -.43；P =.02）。患者脑脊液中L-精氨酸水平低于对照组（P <.001）。地塞米松对NO代谢无显著影响。总之，NO生成增加可能在细菌性脑膜炎的无氧糖酵解及神经损伤中起作用。
