Heinkelein M, Euler-König I, Klinker H, Rückle-Lanz H, Jassoy C
Department of Internal Medicine, and Medical Policlinic, University of Würzburg, Germany.
J Infect Dis. 1996 Jul;174(1):209-13. doi: 10.1093/infdis/174.1.209.
The aim of this study was to investigate the extent of lysis mediated by cytotoxic T lymphocytes (CTL) directed against human immunodeficiency virus (HIV) type 1 gag protein and envelope glycoprotein in peripheral blood mononuclear cells (PBMC) from HIV-1-infected subjects and to compare it with nonspecific envelope glycoprotein-directed cytotoxicity involving CD4 T cells. Most seropositive subjects exhibited antigen-specific cytotoxicity directed at one or both viral antigens in unstimulated or in vitro-stimulated PBMC (or both) mediated by CD8 T cells. In addition, all donors, including seronegative control persons, exhibited nonspecific calcium-independent cytotoxicity involving CD4 T cells and envelope glycoprotein-expressing cells. No calcium-dependent, antigen-specific CD4 T cell-mediated cytolysis was detected. In seropositive subjects, the vigor of nonspecific cytotoxicity was comparable to lysis by antigen-specific CD8 CTL and suggests that it may contribute to lysis of HIV-infected cells in vitro and in vivo.
本研究的目的是调查细胞毒性T淋巴细胞（CTL）介导的针对人类免疫缺陷病毒（HIV）1型gag蛋白和包膜糖蛋白的裂解程度，这些CTL来自HIV-1感染受试者的外周血单核细胞（PBMC），并将其与涉及CD4 T细胞的非特异性包膜糖蛋白介导的细胞毒性进行比较。大多数血清阳性受试者在未刺激或体外刺激的PBMC（或两者）中表现出由CD8 T细胞介导的针对一种或两种病毒抗原的抗原特异性细胞毒性。此外，所有供体，包括血清阴性对照者，都表现出涉及CD4 T细胞和表达包膜糖蛋白的细胞的非特异性钙非依赖性细胞毒性。未检测到钙依赖性、抗原特异性CD4 T细胞介导的细胞溶解。在血清阳性受试者中，非特异性细胞毒性的强度与抗原特异性CD8 CTL的裂解作用相当，这表明它可能在体外和体内对HIV感染细胞的裂解起作用。
