Rosenberg S M, Harris R S, Longerich S, Galloway A M
Department of Biochemistry, University of Alberta Faculty of Medicine, Edmonton, Canada.
Mutat Res. 1996 Feb 19;350(1):69-76. doi: 10.1016/0027-5107(95)00092-5.
Over the past 6 years an unexpected way of making mutations in bacteria has challenged concepts of the genetic mechanisms behind evolution. Mechanistic studies of these so called 'adaptive' mutations are revealing a novel molecular mechanism involving DNA double-strand breaks, genetic recombination, probable DNA polymerase errors, and the possible suspension of mismatch repair during the reversion of a lac frameshift mutation in Escherichia coli. The molecular details of this process are altering our understanding of how mutations form in non-dividing cells.
在过去6年里，一种在细菌中制造突变的意外方式对进化背后遗传机制的概念提出了挑战。对这些所谓“适应性”突变的机制研究正在揭示一种新的分子机制，该机制涉及DNA双链断裂、基因重组、可能的DNA聚合酶错误，以及在大肠杆菌中乳糖移码突变回复过程中错配修复可能的暂停。这一过程的分子细节正在改变我们对非分裂细胞中突变如何形成的理解。
