Nowakowska B
Laboratorium Immunologii Tkankowej.
Postepy Hig Med Dosw. 1995;49(1):107-15.
The main problem in matching donor-recipient pair in kidney transplantations is sensitization of dialysed patients. The patients with high percentage of cytotoxic antibodies to peripheral blood lymphocytes from a random panel, have a low chance of receiving a renal allograft. Negative results of the crossmatch between recipient serum and donor lymphocytes is decisive criterion for kidney transplantation. However not all antibodies are harmful for the graft. We present out methods of differentiating between auto- and alloantibodies and between IgM and IgG class of alloantibodies in sera of hypersensitized patients. The precision of identification of HLA matched donors is vital in bone marrow transplantations. The standard cytotoxicity tests cannot identify all HLA class II alleles and MLC testing is a long procedure. We present our observations from introducing the PCR Fingerprinting technique for a rapid identification of the class II compatible individual among potential donors bone marrow transplant.
肾移植中供体 - 受体配对的主要问题是透析患者的致敏作用。对来自随机样本的外周血淋巴细胞具有高比例细胞毒性抗体的患者，接受肾移植的机会较低。受体血清与供体淋巴细胞之间交叉配型的阴性结果是肾移植的决定性标准。然而，并非所有抗体都对移植物有害。我们介绍了区分超敏患者血清中自身抗体和同种抗体以及同种抗体的IgM和IgG类别的方法。在骨髓移植中，精确识别HLA匹配的供体至关重要。标准细胞毒性试验无法识别所有HLA II类等位基因，且混合淋巴细胞培养试验是一个漫长的过程。我们介绍了引入PCR指纹技术以快速识别潜在供体骨髓移植中II类相容性个体的观察结果。
