Biolo R, Jori G, Soncin M, Rihter B, Kenney M E, Rodgers M A
Department of Biology, University of Padova, Italy. valduga/cribi 1.bio.unipd.it
Photochem Photobiol. 1996 Feb;63(2):224-8. doi: 10.1111/j.1751-1097.1996.tb03018.x.
Previous studies (Biolo et al., Photochem. Photobiol. 59, 362-365, 1994) showed that liposome-delivered Si(IV)-naphthalocyanine (SiNc) photosensitizes B16 pigmented melanoma subcutaneously transplanted in C57 mice to the action of 776 nm light. However, the efficacy of the phototreatment was limited by a lack of selectivity of tumor targeting by SiNc as well as by incomplete necrosis of the neoplastic mass. The present investigations show that the use of a different delivery system (Cremophor emulsion vs liposomes of dipalmitoylphosphatidylcholine) causes no significant increase in the selectivity of tumor targeting for three injected doses of SiNc (0.5, 1, 2 mg/kg). However, upon 776 nm light irradiation (300 mW/cm2; 520 J/cm2), the delay in the rate of tumor growth was maximal (7-8 days) for the highest naphthalocyanine dose. On the other hand, a remarkable improvement in the tumor response was obtained by inducing an intratumoral temperature increase to 44 degrees C immediately after PDT. The thermal effect appeared to be due to photoexcitation of melanin by 776 nm light (550 mW/cm2; 520 J/cm2) and subsequent partial conversion of absorbed energy into heat.
先前的研究（比奥洛等人，《光化学与光生物学》，第59卷，第362 - 365页，1994年）表明，脂质体递送的硅（IV）-萘酞菁（SiNc）可使皮下移植于C57小鼠的B16色素性黑色素瘤对776 nm光的作用产生光致敏。然而，光疗的疗效受到SiNc肿瘤靶向缺乏选择性以及肿瘤块不完全坏死的限制。目前的研究表明，使用不同的递送系统（聚氧乙烯蓖麻油乳剂与二棕榈酰磷脂酰胆碱脂质体）对于三种注射剂量的SiNc（0.5、1、2 mg/kg），并未显著提高肿瘤靶向的选择性。然而，在776 nm光照射（300 mW/cm²；520 J/cm²）后，对于最高萘酞菁剂量，肿瘤生长速率的延迟最大（7 - 8天）。另一方面，通过在光动力疗法（PDT）后立即将肿瘤内温度升至44摄氏度，肿瘤反应得到了显著改善。热效应似乎是由于776 nm光（550 mW/cm²；520 J/cm²）对黑色素的光激发以及随后吸收的能量部分转化为热量所致。
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