非洲爪蟾中停滞的DNA复制及由大肠杆菌诱变蛋白引发的释放

Arrested DNA replication in Xenopus and release by Escherichia coli mutagenesis proteins.

作者信息

Oda N, Levin J D, Spoonde A Y, Frank E G, Levine A S, Woodgate R, Ackerman E J

机构信息

Office of Scientific Director, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Science. 1996 Jun 14;272(5268):1644-6. doi: 10.1126/science.272.5268.1644.

DOI:10.1126/science.272.5268.1644

PMID:8658137

Abstract

Xenopus oocytes and oocyte nuclear extracts repair ultraviolet photoproducts on double-stranded (ds) DNA and replicate single-stranded (ss) to ds DNA. M13 ss DNA molecules containing cyclobutane pyrimidine dimers were maintained but not replicated in Xenopus oocytes yet were replicated in progesterone-matured oocytes. The replication arrest functioned only in cis. The replication arrest was alleviated by injection into oocytes of messenger RNAs encoding the prokaryotic mutagenesis proteins UmuD'C or MucA'B. These results may help explain how cells stabilize repair or replication events on DNA with unrepairable lesions.

摘要

非洲爪蟾卵母细胞和卵母细胞核提取物可修复双链（ds）DNA上的紫外线光产物，并将单链（ss）DNA复制为dsDNA。含有环丁烷嘧啶二聚体的M13单链DNA分子在非洲爪蟾卵母细胞中能维持但不能复制，而在经孕酮处理成熟的卵母细胞中则可复制。复制阻滞仅在顺式作用中起作用。通过向卵母细胞注射编码原核诱变蛋白UmuD'C或MucA'B的信使RNA可缓解复制阻滞。这些结果可能有助于解释细胞如何稳定对具有不可修复损伤的DNA进行的修复或复制事件。