Belozerov V N, Luzev V V
Parazitologiia. 1977 Jan-Feb;11(1):35-42.
The polyacrylamide-gel electrophoresis was used to separate the negative charged proteins from adult females of the tick Dermacentor marginatus Sulz. There were separated up to 25 protein bands from haemolymph and up to 19 bands from midgut extracts of the engorged females. The number of protein bands in excreta and eggs was 15 and 18 respectively. Haemoproteins from haemolymph, midgut and eggs were different in their electrophoretic mobility. Active and diapausing ticks showed essential differences in protein spectra and content of haemolymph (the enrichment of them was characteristic of diapausing ticks). The presence or absence of reproductive diapause affects the digestive functions and has no influence on the excretory functions of the midgut.
采用聚丙烯酰胺凝胶电泳法从边缘革蜱成年雌蜱中分离带负电荷的蛋白质。饱血雌蜱血淋巴中分离出多达25条蛋白带,中肠提取物中分离出多达19条蛋白带。排泄物和卵中的蛋白带数量分别为15条和18条。血淋巴、中肠和卵中的血红蛋白在电泳迁移率上有所不同。活动期蜱和滞育期蜱在血淋巴的蛋白质谱和含量上存在本质差异(滞育期蜱的血淋巴蛋白含量更高)。生殖滞育的存在与否会影响中肠的消化功能,但对中肠的排泄功能没有影响。