Elsukova E I, Medvedev L N, Luzhnov Iu P
Tsitologiia. 1995;37(5-6):440-3.
An attempt was taken to incorporate Ca2(+)-dependent photoprotein obelin into young brown adipocytes using the three methods: 1) osmotic lysis of pinocytotic vesicles; 2) electroporation in a high electric field; 3) hypoosmotic shock. The young adipocytes were isolated from rat interscapular brown fat. The maximum incorporation of obelin into these cells was achieved using the hypoosmotic shock technique. A constant luminescence of intact cells loaded with obelin following hypoosmotic shock was observed in three independent experiments.
1）胞饮小泡的渗透裂解；2）在高电场中进行电穿孔；3）低渗休克。幼年脂肪细胞从大鼠肩胛间棕色脂肪中分离得到。采用低渗休克技术可使水母发光蛋白最大程度地导入这些细胞。在三个独立实验中均观察到，经低渗休克处理后，装载了水母发光蛋白的完整细胞发出恒定的光。
