Yamagishi M, Takami S, Getchell T V
Department of Surgery, University of Kentucky College of Medicine, Lexington 40536-0084, USA.
Ann Otol Rhinol Laryngol. 1996 Feb;105(2):132-9. doi: 10.1177/000348949610500208.
Expression of a calcium-binding protein, spot 35 protein (S-35, calbindin-D28k), was investigated immunohistochemically in the human olfactory mucosa of patients who ranged in age from 16 weeks of fetal development to 98 years old, including some with Alzheimer's disease (AD). S-35 immunoreactivity was observed clearly in olfactory receptor neurons (ORNs) and olfactory nerve bundles that were identified previously with antibodies to olfactory marker protein (OMP) and neuron-specific enolase (NSE). Throughout all ages, the mean number of ORNs immunoreactive for OMP did not change significantly, whereas the mean number of NSE- and S-35-immunoreactive ORNs declined markedly in the postnatal infant, young, and old patients when compared with that of the prenatal fetuses. S-35-immunoreactive ORNs decreased significantly in AD patients when compared with AD control patients. These results indicate that ORNs in humans express S-35 and that there is an age-related trend in the expression of S-35. Furthermore, the marked decrease of S-35 expression in ORNs of AD patients suggests that cell excitability associated with calcium ions and cell protective function against overload of intracellular calcium ions decline in these patients.
采用免疫组织化学方法，对年龄从胎儿发育16周直至98岁的患者的人嗅黏膜中钙结合蛋白即斑点35蛋白（S-35，钙结合蛋白-D28k）的表达情况进行了研究，其中包括一些患有阿尔茨海默病（AD）的患者。在先前用嗅觉标记蛋白（OMP）和神经元特异性烯醇化酶（NSE）抗体鉴定出的嗅觉受体神经元（ORN）和嗅神经束中，清晰观察到了S-35免疫反应性。在所有年龄段中，OMP免疫反应性ORN的平均数没有显著变化，而与产前胎儿相比，出生后的婴儿、年轻患者和老年患者中NSE和S-35免疫反应性ORN的平均数显著下降。与AD对照患者相比，AD患者中S-35免疫反应性ORN显著减少。这些结果表明，人类的ORN表达S-35，并且S-35的表达存在与年龄相关的趋势。此外，AD患者ORN中S-35表达的显著降低表明，这些患者中与钙离子相关的细胞兴奋性以及针对细胞内钙离子过载的细胞保护功能下降。
