更昔洛韦治疗的人巨细胞病毒感染的视网膜神经胶质细胞中复制中间体的维持
Maintenance of replicative intermediates in ganciclovir-treated human cytomegalovirus-infected retinal glia.
作者信息
Burd E M, Pulido J S, Puro D G, O'Brien W J
机构信息
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, USA.
出版信息
Arch Ophthalmol. 1996 Jul;114(7):856-61. doi: 10.1001/archopht.1996.01100140070011.
OBJECTIVES
To characterize the molecular structure of the human cytomegalovirus (HCMV) DNA maintained in cultures of human retinal glia following ganciclovir treatment and to determine the biological activity of the DNA.
METHODS
Cultures of human retinal glia were established, infected with HCMV, treated with ganciclovir, and embedded in agarose, and the viral DNA was analyzed by field inversion gel electrophoresis.
RESULTS
The HCMV DNA was found to persist in cultures of infected, ganciclovir-treated retinal glial cells in the form of replicative intermediates. After removal of ganciclovir, processed forms of DNA in the 500-to 1000-kilobase range were found as well as 230-kb unit length genome. Infectious virus was recovered after termination of ganciclovir treatment.
CONCLUSION
The data are consistent with the concept that ganciclovir's virostatic nature permits maintenance of HCMV DNA in retinal glia in a biologically active form that is capable of replication after removal of the drug.
目的
对经更昔洛韦治疗后在人视网膜神经胶质细胞培养物中维持的人巨细胞病毒（HCMV）DNA的分子结构进行表征，并确定该DNA的生物活性。
方法
建立人视网膜神经胶质细胞培养物，用HCMV感染，用更昔洛韦治疗，然后包埋在琼脂糖中，通过脉冲场凝胶电泳分析病毒DNA。
结果
发现HCMV DNA以复制中间体的形式持续存在于经更昔洛韦治疗的感染视网膜神经胶质细胞培养物中。去除更昔洛韦后，发现了500至1000千碱基范围内的加工形式的DNA以及230千碱基的单位长度基因组。更昔洛韦治疗终止后可回收感染性病毒。
结论
数据与以下概念一致，即更昔洛韦的抑制病毒特性允许HCMV DNA以生物活性形式维持在视网膜神经胶质细胞中，该形式在去除药物后能够复制。
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