脱氢酶与葡萄糖转运蛋白1（GLUT1）信使核糖核酸（mRNA）的3'非翻译区结合。
Dehydrogenase binding to the 3'-untranslated region of GLUT1 mRNA.
作者信息
McGowan K, Pekala P H
机构信息
Department of Biochemistry, East Carolina University, School of Medicine, Greenville, North Carolina 27834, USA.
出版信息
Biochem Biophys Res Commun. 1996 Apr 5;221(1):42-5. doi: 10.1006/bbrc.1996.0541.
Employing RNA gel mobility shift assays we detected specific binding events between several dehydrogenases and various regions of the GLUT1 mRNA 3'-untranslated region. In particular, the enzymes glyceraldehyde 3-phosphate dehydrogenase (G3PDH), lactate dehydrogenase (LDH), and glucose 6-phosphate dehydrogenase (G6PDH) bound to the GLUT1 3'-UTR while isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GluDH) did not. Comparison of shifts obtained with purified dehydrogenases to those obtained using authentic cell extracts indicate that G3PDH and G6PDH may play a role in the intact cell.
通过RNA凝胶迁移率变动分析，我们检测到几种脱氢酶与GLUT1 mRNA 3'-非翻译区的不同区域之间存在特异性结合事件。特别是，甘油醛-3-磷酸脱氢酶（G3PDH）、乳酸脱氢酶（LDH）和葡萄糖-6-磷酸脱氢酶（G6PDH）与GLUT1 3'-UTR结合，而异柠檬酸脱氢酶（IDH）和谷氨酸脱氢酶（GluDH）则不结合。将纯化的脱氢酶获得的迁移结果与使用真实细胞提取物获得的结果进行比较表明，G3PDH和G6PDH可能在完整细胞中发挥作用。
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