Wenger R H, Rolfs A, Marti H H, Guénet J L, Gassmann M
Physiologisches Institut, Universität Zürich-Irchel, Switzerland.
Biochem Biophys Res Commun. 1996 Jun 5;223(1):54-9. doi: 10.1006/bbrc.1996.0845.
The heterodimeric hypoxia-inducible transcription factor HIF-1 is involved in the oxygen-regulated transcription of several genes including erythropoietin. Cloning and sequencing of the alpha-subunit of mouse HIF-1 cDNA revealed a 90% overall homology to human HIF-l alpha but lack of any similarity in the 5' untranslated region and translational start site. Mouse HIF-1 alpha is encoded by an evolutionary conserved single-copy gene located on chromosome 12. We found a widespread constitutive expression of mouse HIf-1 alpha mRNA which was particularly high in lung and kidney. Despite a strong erythropoietin induction, HIF-1 alpha mRNA concentrations were not upregulated in hypoxic mouse tissues.
异二聚体缺氧诱导转录因子HIF-1参与包括促红细胞生成素在内的多个基因的氧调节转录。小鼠HIF-1 cDNAα亚基的克隆和测序显示，其与人类HIF-1α的总体同源性为90%，但在5'非翻译区和翻译起始位点没有任何相似性。小鼠HIF-1α由位于12号染色体上的一个进化保守的单拷贝基因编码。我们发现小鼠HIF-1α mRNA广泛组成性表达，在肺和肾中尤其高。尽管促红细胞生成素诱导强烈，但在缺氧小鼠组织中HIF-1α mRNA浓度并未上调。
