Matucci R, Bianchi B, Ottaviani M F, Campana S, Bennardini F, Franconi F
Department of Preclinical and Clinical Pharmacology, Cystic Fibrosis Center-Meyer Hospital, University of Florence, Italy.
Biochem Biophys Res Commun. 1996 Jun 5;223(1):85-90. doi: 10.1006/bbrc.1996.0850.
The carbon centered radicals generated by preincubation of cumene hydroperoxide with rat cardiac membranes dose dependently reduced the Bmax of [3H]nitrendipine binding, while KD was unchanged. The reduction induced by cumene hydroperoxide was prevented by 2,6-di-t-butlyl-4-methyl-phenol. The generation of OH did not influence the [3H]nitrendipine binding; also other oxidative agents (H2O2 and HClO4) did not modify this binding. Therefore the reduction in [3H]nitrendipine binding sites is not attributable to generic oxidative stress but to the formation of carbon centered radicals.
将异丙苯过氧化氢与大鼠心肌膜预孵育产生的碳中心自由基剂量依赖性地降低了[3H]尼群地平结合的Bmax，而KD不变。2,6-二叔丁基-4-甲基苯酚可阻止异丙苯过氧化氢诱导的降低。OH的产生不影响[3H]尼群地平结合；其他氧化剂（H2O2和HClO4）也未改变这种结合。因此，[3H]尼群地平结合位点的减少并非归因于一般的氧化应激，而是归因于碳中心自由基的形成。
