乳酸克鲁维酵母细胞色素c1基因的分离与分子分析。

Isolation and molecular analysis of the gene for cytochrome c1 from Kluyveromyces lactis.

作者信息

Gbelská Y, Horváthová K, van der Aart Q J, Zonneveld B J, Steensma H Y, Subík J

机构信息

Department of Microbiology and Virology, Comenius University, Mlynská dolina B-2, 842 15 Bratislava, Slovak Republic.

出版信息

Curr Genet. 1996 Jul 31;30(2):145-50. doi: 10.1007/s002940050113.

DOI:10.1007/s002940050113

PMID:8660461

Abstract

By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of these mutants we have cloned the structural gene for cytochrome c1, named KlCYT1. This gene has been assigned to chromosome VI and its nucleotide sequence exhibited 74.3% identity to the homologous gene of S. cerevisiae.

摘要

通过对乳酸克鲁维酵母进行甲磺酸乙酯诱变，我们分离出了五个在非发酵性碳源上无法生长的核突变体。这些突变被发现属于三个互补群。在对其中一个突变体的突变进行功能互补后，我们克隆了细胞色素c1的结构基因，命名为KlCYT1。该基因已被定位到第六条染色体上，其核苷酸序列与酿酒酵母的同源基因具有74.3%的同一性。