Sarinová M, Straková V, Balková K, Gbelská Y
Department of Microbiology and Virology, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
Folia Microbiol (Praha). 2007;52(5):484-90. doi: 10.1007/BF02932108.
Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.
与同基因野生型菌株相比，观察到细胞色素c、细胞色素c1和细胞色素c氧化酶功能受损的乳酸克鲁维酵母突变体对氟康唑、联苯苄唑和两性霉素B的敏感性降低。用罗丹明6G进行的流式细胞术分析表明，与相应的野生型菌株相比，突变体细胞中染料的积累没有任何变化。甾醇分析显示野生型和突变体细胞中甾醇的总量相似。考虑到突变体细胞对两性霉素B的抗性增加以及对溶菌酶消化的敏感性显著降低，细胞壁结构和/或组成可能是观察到的突变体对所用抗真菌化合物敏感性变化的原因。
