Milisavljevic V, Freedberg I M, Blumenberg M
The Ronald O. Perelman Department of Dermatology, New York University Medical Center, 550 First Avenue, New York, New York, 10016, USA.
Genomics. 1996 May 15;34(1):134-8. doi: 10.1006/geno.1996.0252.
Mapping studies of functional keratin genes in the human genome have localized most of the acidic keratin genes to chromosome 17q12-q21 and the basic keratin genes to chromosome 12q11-q13. Within the acidic keratin locus two clusters were identified, one containing the genes for K15 and K19, the other the genes for K14, K16, and K17. The relative positions and the distance between the two clusters have not been determined previously. In this paper we describe our analysis of P1 clones containing multiple acidic keratin genes, which were studied using restriction analysis and Southern blot hybridization with PCR-amplified probes specific for functional human keratin genes 15, 17, and 19. Our results show that the two clusters are very closely linked to each other, within a 55-kb region in the human genome. The genes are organized 5' to 3' in the following order: 5'-K19-K15-K17-K16-K14. Between K15 and K17 at least one additional, unidentified keratin gene is present.
对人类基因组中功能性角蛋白基因的定位研究已将大多数酸性角蛋白基因定位于17号染色体q12 - q21区域，将碱性角蛋白基因定位于12号染色体q11 - q13区域。在酸性角蛋白基因座内鉴定出两个基因簇，一个包含K15和K19基因，另一个包含K14、K16和K17基因。此前尚未确定这两个基因簇的相对位置和它们之间的距离。在本文中，我们描述了对含有多个酸性角蛋白基因的P1克隆的分析，使用限制性分析以及与针对功能性人类角蛋白基因15、17和19的PCR扩增探针进行Southern印迹杂交。我们的结果表明，这两个基因簇在人类基因组中一个55 kb的区域内彼此紧密连锁。这些基因按5'至3'的顺序排列如下：5'-K19-K15-K17-K16-K14。在K15和K17之间至少存在一个额外的、未鉴定的角蛋白基因。
