Wagner B J, Long L, Rao P N, Pettenati M J, Brewer G
Department of Microbiology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina, 27157, USA.
Genomics. 1996 Jun 1;34(2):219-22. doi: 10.1006/geno.1996.0269.
Messenger RNAs encoding many oncoproteins and cytokines are relatively unstable. Their instability, which ensures appropriate levels and timing of expression, is controlled in part by proteins that bind to A+U-rich instability elements (AREs) present in the 3'-untranslated regions of the mRNAs. cDNAs encoding the AUF1 family of ARE-binding proteins were cloned from human and murine cDNA libraries. In the present study monochromosomal somatic cell hybrids were used to localize two AUF1 loci to human chromosomes 4 and X. In situ hybridization analyses using P1 clones as probes identified the 4q21.1-q21.2 and Xq12 regions as the locations of the AUF1 genes.
编码许多癌蛋白和细胞因子的信使核糖核酸相对不稳定。它们的不稳定性确保了适当的表达水平和时间，部分受与富含A+U的不稳定元件（AREs）结合的蛋白质控制，这些元件存在于信使核糖核酸的3'非翻译区。从人和小鼠的cDNA文库中克隆了编码ARE结合蛋白AUF1家族的cDNA。在本研究中，使用单染色体体细胞杂种将两个AUF1基因座定位到人类染色体4和X上。使用P1克隆作为探针的原位杂交分析确定4q21.1-q21.2和Xq12区域为AUF1基因的位置。
