Arai Y, Hosoda F, Nakayama K, Ohki M
Radiobiology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104, Japan.
Genomics. 1996 Jul 1;35(1):196-206. doi: 10.1006/geno.1996.0339.
Human chromosome 11q22-q23 is a pathologically important region in which a high level of loss of heterozygosity has been reported for breast, ovary, cervical, colon, and lung carcinomas, malignant melanomas, and hematologic malignancies. This strongly indicates that one or more tumor suppressor genes reside within the deleted region. In this report, we report the development of a contig map that covers most of the deleted regions found in these malignancies. The map comprises a contig of 66 overlapping yeast artificial chromosomes (YACs) and spans a region of 17 Mb from the PGR gene at 11q22.2 to the MLL gene at q23.3. In the process of screening the YACs, 50 new sequence-tagged site markers were developed from the termini of the YAC inserts. These markers were used for chromosome walking, and the data were then integrated into the contig map. NotI restriction mapping of these YACs revealed the presence of at least 26 NotI sites in the region. Using 22 of them, a NotI restriction map of the region from PGR to D11S939 was developed. This YAC contig will provide efficient tools for identification of the putative tumor suppressor gene(s).
人类染色体11q22 - q23是一个具有重要病理学意义的区域，据报道，在乳腺癌、卵巢癌、宫颈癌、结肠癌、肺癌、恶性黑色素瘤和血液系统恶性肿瘤中，该区域存在高水平的杂合性缺失。这强烈表明一个或多个肿瘤抑制基因位于该缺失区域内。在本报告中，我们报道了一个覆盖这些恶性肿瘤中发现的大部分缺失区域的重叠群图谱的构建。该图谱由66个重叠的酵母人工染色体（YAC）组成的重叠群构成，跨度为17 Mb，从11q22.2的PGR基因到q23.3的MLL基因。在筛选YAC的过程中，从YAC插入片段的末端开发了50个新的序列标签位点标记。这些标记用于染色体步移，然后将数据整合到重叠群图谱中。对这些YAC进行NotI酶切图谱分析显示该区域至少存在26个NotI位点。利用其中22个位点，构建了从PGR到D11S939区域的NotI酶切图谱。这个YAC重叠群将为鉴定假定的肿瘤抑制基因提供有效的工具。
