利用体细胞杂种定位板对人类22号染色体上300多个基因座进行区域定位。

Regional localization of over 300 loci on human chromosome 22 using a somatic cell hybrid mapping panel.

作者信息

Budarf M L, Eckman B, Michaud D, McDonald T, Gavigan S, Buetow K H, Tatsumura Y, Liu Z, Hilliard C, Driscoll D, Goldmuntz E, Meese E, Zwarthoff E C, Williams S, McDermid H, Dumanski J P, Biegel J, Bell C J, Emanuel B S

机构信息

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA.

出版信息

Genomics. 1996 Jul 15;35(2):275-88. doi: 10.1006/geno.1996.0358.

DOI:10.1006/geno.1996.0358

PMID:8661140

Abstract

A somatic cell hybrid panel, consisting of 25 cell lines, has been developed to localize loci subregionally on chromosome 22. Over 300 markers in the form of STSs or hybridization probes have been assigned to one of 24 unique regions or "bins" using this panel. This ordered collection of markers will aid in the assembly of physical maps and contigs of chromosome 22 and assist in positional cloning of disease loci mapped to chromosome 22.

摘要

一个由25个细胞系组成的体细胞杂交板已被开发出来，用于在22号染色体上进行区域定位。利用该杂交板，超过300个以序列标签位点（STS）或杂交探针形式存在的标记已被定位到24个独特区域或“间隔区”中的一个。这种有序排列的标记集合将有助于构建22号染色体的物理图谱和重叠群，并协助对定位到22号染色体上的疾病位点进行位置克隆。