Overhauser J, Mewar R, Rojas K, Lia K, Kline A D, Silverman G A
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Genomics. 1993 Feb;15(2):387-91. doi: 10.1006/geno.1993.1072.
Somatic cell hybrids containing different deleted regions of chromosome 18 derived from patients with balanced translocations or terminal deletions were used to create a deletion mapping panel. Twenty-four sequence-tagged sites (STSs) for 17 genes and 7 anonymous polymorphic DNA fragments were identified. These STSs were used to map the 24 loci to 18 defined regions of chromosome 18. Both ERV1, previously mapped to 18q22-q23, and YES1, previously mapped to 18q21.3, were found to map to 18q11.21-pter. Several genes previously mapped to 18q21 were found to be in the order cen-SSAV1-DCC-FECH-GRP-BCL2-PLANH2-tel. The precise mapping of genes to chromosome 18 should help in determining whether these genes may be involved in the etiology of specific chromosomal syndromes associated with chromosome 18. The mapping of the polymorphic loci will assist in the integration of the physical map with the recombination map of chromosome 18.
含有来自平衡易位或末端缺失患者的18号染色体不同缺失区域的体细胞杂种被用于构建一个缺失定位板。鉴定了17个基因的24个序列标签位点（STS）和7个匿名多态性DNA片段。这些STS被用于将24个基因座定位到18号染色体的18个定义区域。先前定位于18q22 - q23的ERV1和先前定位于18q21.3的YES1均被发现定位于18q11.21 - pter。先前定位于18q21的几个基因的顺序为cen - SSAV1 - DCC - FECH - GRP - BCL2 - PLANH2 - tel。将基因精确地定位到18号染色体应有助于确定这些基因是否可能参与与18号染色体相关的特定染色体综合征的病因。多态性基因座的定位将有助于将物理图谱与18号染色体的重组图谱整合。
