Fouchécourt M O, Rivière J L
Institut National de la Recherche Agronomique, Unité Associée de Toxicologie Métabolique et d'Ecotoxicologie INRA-DGER, France.
Arch Environ Contam Toxicol. 1996 May;30(4):513-22. doi: 10.1007/BF00213403.
Laboratory and wild Norway rats were exposed in the laboratory to an uncontaminated soil and to a soil from a site contaminated with petrochemical waste. Activities of microsomal lung and liver cytochrome P450-dependent monooxygenases, including 7-ethoxyresorufin O-deethylase (EROD), 7-pentoxyresorufin O-depentylase (PROD) and 7-benzoxyresorufin O-debenzylase (BROD) were measured at selected times during the course of the study. The highest degree of induction of hepatic EROD (7-fold) was shown after 3 days of exposure to the contaminated soil. However, two months later, the EROD activity declined to fourfold increase over the control. The PROD and BROD activities displayed a similar time course of induction, but the degree of induction was lower. The induction of hepatic monooxygenase activities was observed in both laboratory and wild rats. Lung monooxygenase EROD was highly induced (up to 28-fold) after 3 days of exposure, and the activity remained elevated throughout the two-month experiment. BROD and PROD activities were not induced. The activities of three antioxidant enzymes, namely superoxide dismutase, glutathione peroxidase (Se- and non-Se- dependent) and catalase also were measured in lung and liver cytosol, but no significant changes were observed after two months of exposure to contaminated soil.
在实验室中，将实验用挪威大鼠和野生挪威大鼠分别暴露于未受污染的土壤以及受石化废物污染场地的土壤中。在研究过程中的选定时间，测定了微粒体肺和肝细胞色素P450依赖性单加氧酶的活性，包括7-乙氧基异吩唑酮O-脱乙基酶（EROD）、7-戊氧基异吩唑酮O-脱戊基酶（PROD）和7-苯氧基异吩唑酮O-脱苄基酶（BROD）。暴露于受污染土壤3天后，肝脏EROD的诱导程度最高（7倍）。然而，两个月后，EROD活性下降至比对照组增加四倍。PROD和BROD活性呈现出相似的诱导时间进程，但诱导程度较低。在实验用大鼠和野生大鼠中均观察到肝脏单加氧酶活性的诱导。暴露3天后，肺单加氧酶EROD被高度诱导（高达28倍），并且在整个两个月的实验过程中活性一直保持升高。BROD和PROD活性未被诱导。还测定了肺和肝细胞溶胶中三种抗氧化酶的活性，即超氧化物歧化酶、谷胱甘肽过氧化物酶（硒依赖性和非硒依赖性）和过氧化氢酶，但暴露于受污染土壤两个月后未观察到显著变化。
