Fouchécourt M O, Rivière J L
Institut National de la Recherche Agronomique, Unité associée INRA-ENVL de Toxicologie Métabolique et d'Ecotoxicologie, Marcy l'Etoile, France.
Chemosphere. 1995 Dec;31(11-12):4375-86. doi: 10.1016/0045-6535(95)00305-r.
Wild Norway rats (Rattus norvegicus) were collected from a site contaminated by a range of polycyclic aromatic hydrocarbons (PAHs), mineral oils, polychlorobiphenyls (PCBs) and heavy metals. Activities of cytochrome P450 monooxygenases (ethoxy-, pentoxy- and benzoxy-resorufin O-dealkylases, and 4-nitrophenol hydroxylase) were measured in microsomal fractions from liver and lung. Antioxidant enzyme activities (superoxide dismutase, catalase, selenium-dependent and non-selenium-dependent glutathione peroxidases) were also measured in cytosolic fractions from lung and liver, and in erythrocytes. The levels of activities were compared with those found in control laboratory rats and in wild Norway rats reared in a terrarium. Results show that rats living in a polluted environment have monooxygenase activities higher than that of control animals in both liver and lung. Some modifications of antioxidant enzyme activities were also found in these animals.
野生挪威大鼠（褐家鼠）采自一个受到多种多环芳烃（PAHs）、矿物油、多氯联苯（PCBs）和重金属污染的地点。在肝脏和肺脏的微粒体部分测定了细胞色素P450单加氧酶（乙氧基、戊氧基和苯氧基-试卤灵O-脱烷基酶，以及4-硝基苯酚羟化酶）的活性。还在肺脏和肝脏的胞质部分以及红细胞中测定了抗氧化酶活性（超氧化物歧化酶、过氧化氢酶、硒依赖性和非硒依赖性谷胱甘肽过氧化物酶）。将这些活性水平与在对照实验室大鼠和饲养在玻璃容器中的野生挪威大鼠中发现的活性水平进行了比较。结果表明，生活在污染环境中的大鼠肝脏和肺脏中的单加氧酶活性均高于对照动物。在这些动物中还发现了抗氧化酶活性的一些变化。
