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胚胎鸡脑微粒体部分中的内源性DNA指导的DNA合成系统。

Endogenous DNA-directed DNA synthesising system in a microsomal fraction of embryonic chick brain.

作者信息

Smith J, Soriano L

出版信息

Nucleic Acids Res. 1977 Mar;4(3):641-8. doi: 10.1093/nar/4.3.641.

DOI:10.1093/nar/4.3.641
PMID:866184
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342468/
Abstract

A DNA polymerising complex directed by endogenous DNA has been partially purified from 11-day-old embryonic chick brain microsomes by DEAE-cellulose and phosphocellulose column chromatography. The active fractions are eluted together with an exogenous DNA-directed DNA polymerase; after Sephadex gel filtration, the endogenous activity remains associated with a high molecular weight DNA-directed DNA polymerase. The endogenous activity of the complex has been shown to be RNase-resistant and actinomycin-sensitive. It requires potassium, an ATP-regenerating system and all four deoxyribonucleoside triphosphates for full activity. The significance of this activity with regard to the protovirus hypothesis is discussed.

摘要

通过二乙氨基乙基纤维素（DEAE -纤维素）和磷酸纤维素柱层析法，已从11日龄鸡胚脑微粒体中部分纯化出一种由内源性DNA指导的DNA聚合酶复合物。活性组分与外源性DNA指导的DNA聚合酶一起被洗脱；经葡聚糖凝胶过滤后，内源性活性仍与一种高分子量的DNA指导的DNA聚合酶相关联。已证明该复合物的内源性活性对核糖核酸酶（RNase）具有抗性，而对放线菌素敏感。它需要钾、一个ATP再生系统以及所有四种脱氧核糖核苷三磷酸才能发挥完全活性。本文讨论了这种活性对于前病毒假说的意义。

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本文引用的文献

1
HOMOLOGY BETWEEN RNA FROM ROUS SARCOMA VIROUS AND DNA FROM ROUS SARCOMA VIRUS-INFECTED CELLS.劳氏肉瘤病毒的RNA与劳氏肉瘤病毒感染细胞的DNA之间的同源性。
Proc Natl Acad Sci U S A. 1964 Aug;52(2):323-9. doi: 10.1073/pnas.52.2.323.
2
DNA polymerase activities in brain of chick embryo.
FEBS Lett. 1972 May 15;22(3):310-314. doi: 10.1016/0014-5793(72)80258-8.
3
Forms of deoxyribonucleic acid produced by virions of the ribonucleic acid tumor viruses.由核糖核酸肿瘤病毒的病毒粒子产生的脱氧核糖核酸形式。
J Virol. 1971 Jan;7(1):106-11. doi: 10.1128/JVI.7.1.106-111.1971.
4
A chemical model for transcriptional initiation of DNA replication.DNA复制转录起始的化学模型。
Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354-60. doi: 10.1016/s0006-291x(72)80124-4.
5
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.RNA聚合酶在M13 DNA合成起始过程中的可能作用。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9. doi: 10.1073/pnas.68.11.2826.
6
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.RNA合成引发M13 DNA在体外转化为其复制形式。
Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9. doi: 10.1073/pnas.69.4.965.
7
Mitochondrial DNA polymerase, deoxyribonuclease and ribonuclease H activities from brain of chick embryo.鸡胚大脑中的线粒体DNA聚合酶、脱氧核糖核酸酶和核糖核酸酶H活性
Nucleic Acids Res. 1974 Sep;1(9):1085-97. doi: 10.1093/nar/1.9.1085.
8
RNA-primed DNA synthesis in vitro.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1560-4. doi: 10.1073/pnas.69.6.1560.
9
Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos.未感染鸡胚中的内源性RNA指导的DNA聚合酶活性
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1550-4. doi: 10.1073/pnas.69.6.1550.
10
Ribonuclease-sensitive deoxyribonucleic acid polymerase activity in uninfected rat cells and rat cells infected with Rous sarcoma virus.未感染的大鼠细胞和感染劳氏肉瘤病毒的大鼠细胞中对核糖核酸酶敏感的脱氧核糖核酸聚合酶活性
J Virol. 1971 Nov;8(5):630-42. doi: 10.1128/JVI.8.5.630-642.1971.

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