Wang F S, Wolenski J S, Cheney R E, Mooseker M S, Jay D G
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
Science. 1996 Aug 2;273(5275):660-3. doi: 10.1126/science.273.5275.660.
The molecular mechanisms underlying directed motility of growth cones have not been determined. The role of myosin-V, an unconventional myosin, in growth cone dynamics was examined by chromophore-assisted laser inactivation (CALI). CALI of purified chick brain myosin-V absorbed onto nitrocellulose-coated cover slips inhibited the ability of myosin-V to translocate actin filaments. CALI of myosin-V in growth cones of chick dorsal root ganglion neurons resulted in rapid filopodial retraction. The rate of filopodial extension was significantly decreased, whereas the rate of filopodial retraction was not affected, which suggests a specific role for myosin-V in filopodial extension.
生长锥定向运动的分子机制尚未确定。通过发色团辅助激光失活（CALI）研究了非传统肌球蛋白肌球蛋白-V在生长锥动力学中的作用。吸附在硝酸纤维素包被盖玻片上的纯化鸡脑肌球蛋白-V的CALI抑制了肌球蛋白-V转运肌动蛋白丝的能力。鸡背根神经节神经元生长锥中肌球蛋白-V的CALI导致丝状伪足迅速回缩。丝状伪足延伸速率显著降低，而丝状伪足回缩速率不受影响，这表明肌球蛋白-V在丝状伪足延伸中具有特定作用。
