Hailey J L, Ingham E, Stone M, Wroblewski B M, Fisher J
Department of Mechanical Engineering, University of Leeds.
Proc Inst Mech Eng H. 1996;210(1):3-10. doi: 10.1243/PIME_PROC_1996_210_385_02.
The objective of this study was to investigate the effect of counterface roughness and lubricant on the morphology of ultra-high molecular weight polyethylene (UHMWPE) wear debris generated in laboratory wear tests, and to compare this with debris isolated from explanted tissue. Laboratory tests used UHMWPE pins sliding against stainless steel counterfaces. Both water and serum lubricants were used in conjunction with rough and smooth counterfaces. The lubricants and tissue from revision hip surgery were processed to digest the proteins and permit filtration. This involved denaturing the proteins with potassium hydroxide (KOH), sedimentation of any remaining proteins, and further digestion of these proteins with chromic acid. All fractions were then passed through a 0.2 micron membrane, and the debris examined using scanning electron microscopy. The laboratory studies showed that the major variable influencing debris morphology was counterface roughness. The rougher counterfaces produced larger numbers of smaller particles, with a size range extending below 1 micron. For smooth counterfaces there were fewer of these small particles, and evidence of larger platelets, greater than 10 microns in diameter. Analysis of the debris from explanted tissues showed a wide variation in the particle size distribution, ranging from below 1 micron up to several millimetres in size. Of major clinical significance in relation to osteolysis and loosening is roughening of the femoral components, which may lead to greater numbers of the sub-micron-sized particles.
本研究的目的是调查配对表面粗糙度和润滑剂对实验室磨损试验中产生的超高分子量聚乙烯（UHMWPE）磨损碎屑形态的影响，并将其与从植入组织中分离出的碎屑进行比较。实验室试验使用UHMWPE销在不锈钢配对表面上滑动。水和血清润滑剂均与粗糙和光滑的配对表面一起使用。对翻修髋关节手术中的润滑剂和组织进行处理，以消化蛋白质并进行过滤。这包括用氢氧化钾（KOH）使蛋白质变性、沉淀任何残留的蛋白质，并用铬酸进一步消化这些蛋白质。然后将所有级分通过0.2微米的膜，并使用扫描电子显微镜检查碎屑。实验室研究表明，影响碎屑形态的主要变量是配对表面粗糙度。较粗糙的配对表面产生大量较小的颗粒，尺寸范围延伸至1微米以下。对于光滑的配对表面，这些小颗粒较少，并且有直径大于10微米的较大血小板的迹象。对植入组织碎屑的分析表明，颗粒大小分布差异很大，范围从1微米以下到几毫米。与骨溶解和松动相关的主要临床意义在于股骨部件的粗糙化，这可能导致更多的亚微米级颗粒。
