Aoki I, Aoki A, Klinman D M
Center for Biologics Evaluation and Research, Food and Drug Administration, USA.
Cytokine. 1995 Nov;7(8):799-805. doi: 10.1006/cyto.1995.0096.
The number, antigenic specificity and phenotype of cells secreting IL-4 and IL-6 in mice immunized with ovalbumin or keyhole limpet haemocyanin (KLH) in Freund's adjuvant (FA) was studied. The frequency of cells producing either of these cytokines began to rise 6 days post immunization, peaked at 11-14 days post-immunization, and fell to background by 21 days. The number of spleen cells secreting IL-6 was higher than the number producing IL-4 at all time points. Boosting elicited an anamnestic response characterized by a significant increase in the number of cytokine secreting cells within 4 days. Cytokine production was induced in multiple strains of normal mice, and was critically dependent on the use of Complete FA in addition to antigen. Immunization induced IL-4 and IL-6 production in vivo while 'priming' additional cells to release these cytokines when reexposed to soluble antigen in vitro. The latter response was antigen specific and was dominated by non-B/non-T cells. Those cells may serve to boost the immune response in cases of persistent or repeated antigenic challenge.
研究了用弗氏佐剂（FA）中的卵清蛋白或钥孔戚血蓝蛋白（KLH）免疫的小鼠中分泌白细胞介素-4（IL-4）和白细胞介素-6（IL-6）的细胞数量、抗原特异性和表型。产生这两种细胞因子中任一种的细胞频率在免疫后6天开始上升，在免疫后11 - 14天达到峰值，并在21天时降至背景水平。在所有时间点，分泌IL-6的脾细胞数量均高于分泌IL-4的细胞数量。加强免疫引发了一种回忆反应，其特征是在4天内细胞因子分泌细胞数量显著增加。细胞因子的产生在多株正常小鼠中均可诱导，并且除抗原外还严重依赖于使用完全弗氏佐剂。免疫在体内诱导IL-4和IL-6的产生，同时“致敏”额外的细胞，使其在体外再次暴露于可溶性抗原时释放这些细胞因子。后一种反应是抗原特异性的，并且以非B/非T细胞为主。在持续或反复抗原刺激的情况下，这些细胞可能有助于增强免疫反应。
