氮氧化物对玻璃工匠和黄铜匠体内自然杀伤细胞的影响。

Effects of nitrogen oxides on natural killer cells in glass craftsmen and braziers.

作者信息

Azari M R, Williams F M, Kirby J, Kelly P, Edwards J W, Blain P G

机构信息

Department of Environmental and Occupational Medicine, Newcastle University, Newcastle Upon Tyne.

出版信息

Occup Environ Med. 1996 Apr;53(4):248-51. doi: 10.1136/oem.53.4.248.

DOI:10.1136/oem.53.4.248

PMID:8664962

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1128458/

Abstract

OBJECTIVES

To assess the effect of exposure to nitrogen oxides on peripheral blood natural killer cells.

METHODS

Groups of glass craftsmen and braziers exposed to nitrogen oxides and non-exposed controls were studied. Air concentrations of nitrogen oxides were measured. Mononuclear cells isolated from peripheral blood samples were assayed for natural killer cell activity with K562 target cells in a 51Cr release assay and the percentage of natural killer cells (CD16) was measured by flow cytometry.

RESULTS

Braziers were exposed to 1.2 ppm nitrogen dioxide and 8.6 ppm nitric oxide and glass craftsmen to 2.9 ppm nitrogen dioxide and 26.5 ppm nitric oxide. The natural killer cell activity of exposed workers was significantly lower than in non-exposed controls (P < 0.05 ANOVA Scheffe test). The percentage of natural killer cells in glass craftsmen was significantly greater than in controls (P < 0.05 ANOVA Scheffe test). Regression of natural killer cell activity against age, smoking habit, number of years worked and current exposure to nitrogen dioxide and nitric oxide gases was not significant. The percentage of natural killer cells was not significantly correlated with age, smoking habit, or numbers of years worked, but was significantly related to air concentrations of nitrogen dioxide (P < 0.01) and nitric oxide (P < 0.001).

CONCLUSION

Natural killer cell activity and the percentage of natural killer cells in peripheral blood cells were altered in workers exposed to nitrogen oxides.

摘要

目的

评估接触氮氧化物对外周血自然杀伤细胞的影响。

方法

对接触氮氧化物的玻璃工匠和黄铜匠群体以及未接触的对照组进行研究。测量空气中氮氧化物的浓度。从外周血样本中分离出的单核细胞，在51Cr释放试验中用K562靶细胞检测自然杀伤细胞活性，并通过流式细胞术测量自然杀伤细胞（CD16）的百分比。

结果

黄铜匠接触到1.2 ppm的二氧化氮和8.6 ppm的一氧化氮，玻璃工匠接触到2.9 ppm的二氧化氮和26.5 ppm的一氧化氮。接触组工人的自然杀伤细胞活性显著低于未接触组对照组（P < 0.05，方差分析Scheffe检验）。玻璃工匠中自然杀伤细胞的百分比显著高于对照组（P < 0.05，方差分析Scheffe检验）。自然杀伤细胞活性与年龄、吸烟习惯、工作年限以及当前接触二氧化氮和一氧化氮气体之间的回归不显著。自然杀伤细胞的百分比与年龄、吸烟习惯或工作年限无显著相关性，但与空气中二氧化氮（P < 0.01）和一氧化氮（P < 0.001）的浓度显著相关。